Tosoh TSKgel UP-SW3000
TSKgel UP-SW3000 UHPLC columns for SEC
Tosoh Bioscience GmbH announces the much-anticipated release of the 2 µm particle size TSKgel UP-SW3000 UHPLC columns for aqueous SEC. These columns are an addition to the successful TSKgel SW series and are especially suited for use with UHPLC equipment. They feature the same pore size as TSKgel G3000SWXL and therefore facilitate the method transfer from G3000SWXL to UHPLC. The key features of TSKgel UP-SW3000 are:
- 2 micron particle size
- 25 nm/250 A pore size, ideal for antibody QC analysis
- Provides the proven TSKgel SW/SWXL/SuperSW series quality for UHPLC users
- Allows an easy transfer of existing methods from HPLC to UHPLC
- 15 cm column speeds up analysis while maintaining resolution (compared to G3000SWXL/HPLC)
- 30 cm column remarkably increases resolution (compared to G3000SWXL/HPLC)
Columns are available in two length, 15 and 30 cm. To avoid extra column peak broadening the direct connect (DC) guard column is recommended. A conventional guard column is available as well.
for pricing please contact our specialists with the contactform on this page.
Protein calibration curve for TSKgel UP-SW3000 and competitor SEC column
Column: TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 15 cm
Competitor SEC, 1.7 µm, 4.6 mm ID × 15 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7
+ 100 mmol/L Na2SO4 + 0.05% NaN3
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Inj. vol.: 5 μL
Samples: 1. thyroglobulin, 640 kDa
1’. thyroglobulin dimer
2. gamma-globulin, 155 kDa
2’. gamma-globulin dimer
3. ovalbumin, 47 kDa
4. ribonuclease A, 13,700 Da
5. p-amino benzoic acid, 137 Da